Journal: Oncology Reports

Article Title: Knockdown of CCT2 inhibits the malignant progression of hepatocellular carcinoma cells by impairing STAT3 activation

doi: 10.3892/or.2026.9086

Figure Lengend Snippet: Knockdown of CCT2 inhibits STAT3 signaling activation in hepatocellular carcinoma cells. The protein levels of STAT3, p-STAT3, MCL1, MMP2 and SOX2 in (A) Huh-7 and (B) HCCLM3 cells were measured by western blotting. The protein levels of (C) p-STAT3, (D) MCL1, (E) MMP2 and (F) SOX2 in subcutaneous tumor tissue were detected by immunohistochemical staining. *P<0.05, **P<0.01 vs. sh-NC. CCT2, chaperonin containing TCP1 subunit 2; sh, short hairpin; NC, negative control; p, phosphorylated; MCL1, myeloid cell leukemia sequence 1; SOX2, SRY-box transcription factor 2.

Article Snippet: The primary antibodies were as follows: CCT2 (cat. no. 24896-1-AP), β-actin (cat. no. 66009-1-Ig), MMP2 (cat. no. 10373-2-AP), myeloid cell leukemia sequence 1 (MCL1; cat. no. 16225-1-AP) and SRY-box transcription factor 2 (SOX2; cat. no. 11064-1-AP; all Proteintech Group, Inc.) and STAT3 (cat. no. 4904) and phosphorylated (p-)STAT3 (Tyr705; cat. no. 4113; both Cell Signaling Technology, Inc.) The membranes were washed three times in TBST (0.1% Tween-20) for 5 min each at room temperature.

Techniques: Knockdown, Activation Assay, Western Blot, Immunohistochemical staining, Staining, Negative Control, Sequencing

Journal: STAR Protocols

Article Title: Protocol to study adult neurogenesis in fresh-frozen human hippocampal tissue using an immunofluorescence quantitative approach

doi: 10.1016/j.xpro.2025.104344

Figure Lengend Snippet: Overview of expected outcomes (A) Schematic representation of distinct cell populations related to adult hippocampal neurogenesis (AHN). (B–H) Expected staining with anti- (B) vimentin, (C) sex determining region Y-box 2 (Sox2), (D) phospho-histone 3 (PH3), (E) HuC-HuD, (F) Doublecortin (DCX), (G) polysialylated-neural cell adhesion molecule (PSA-NCAM), and (H) calbindin (CB) antibodies. (I) Schematic representation of the components of the human hippocampal neurogenic niche. (J–M) Expected staining with anti- (J) S100 calcium-binding protein β (S100β), (K) ionized calcium-binding adaptor molecule 1 (Iba1), (L) phosphorylated γH2A.X (γH2A.X) antibodies, and (M) Ulex Europaeus Agglutinin-I (UEA-1). Yellow scale bar: 20 μm. White scale bar: 10 μm. Yellow triangles indicate positive cells. White triangles highlight the morphology of cells labelled for each marker.

Article Snippet: Goat polyclonal anti-SRY (sex-determining region Y)-box 2 (Sox2) (1:500) , R and D Systems , Cat# AF2018; RRID: AB_355110.

Techniques: Staining, Binding Assay, Marker